Composite

Part:BBa_K3766104:Design

Designed by: Kamyar Keshavarz-Farajkhah   Group: iGEM21_Evry_Paris-Saclay   (2021-09-25)


pBad TadA* T7RNAP CGG-R12-KIRV


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1218
    Illegal PstI site found at 1775
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal PstI site found at 1775
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1218
    Illegal PstI site found at 1775
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1218
    Illegal PstI site found at 1775
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1259
    Illegal AgeI site found at 1604
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

to do


Source

DNA synthesis, PCR and assembly

References